| Registro completo |
| Provedor de dados: |
Anais da ABC (AABC)
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| País: |
Brazil
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| Título: |
Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei , Mytilidae) in environmental samples
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| Autores: |
PIE,MARCIO R.
STRÖHER,PATRÍCIA R.
AGOSTINIS,ANDRÉ O.
BELMONTE-LOPES,RICARDO
TADRA-SFEIR,MICHELLE Z.
OSTRENSKY,ANTONIO
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| Data: |
2017-06-01
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| Ano: |
2017
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| Palavras-chave: |
EDNA
Invasive species
Environmental DNA
Molecular identification
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| Resumo: |
ABSTRACT The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the abundance of the golden mussel in freshwater habitats, particularly in early stages of colonization. In this study, we describe a highly-sensitive real-time PCR assay targeting a 100-bp region of the COI mitochondrial gene of the golden mussel. The method was able to detect as little as 0.225 pg of target DNA. This assay represents an important contribution to surveillance methods, as well as to optimize field measures to contain and manage populations of the golden mussel in its introduced range.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301041
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| Editor: |
Academia Brasileira de Ciências
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| Relação: |
10.1590/0001-3765201720160723
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| Formato: |
text/html
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| Fonte: |
Anais da Academia Brasileira de Ciências v.89 n.2 2017
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| Direitos: |
info:eu-repo/semantics/openAccess
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