| Registro completo |
| Provedor de dados: |
Anais da ABC (AABC)
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| País: |
Brazil
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| Título: |
rDNA mapping, heterochromatin characterization and AT/GC content of Agapanthus africanus (L.) Hoffmanns (Agapanthaceae)
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| Autores: |
REIS,ARYANE C.
FRANCO,ANA LUIZA
CAMPOS,VICTÓRIA R.
SOUZA,FLÁVIA R.
ZORZATTO,CRISTIANE
VICCINI,LYDERSON F.
SOUSA,SAULO M.
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| Data: |
2016-01-01
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| Ano: |
2016
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| Palavras-chave: |
African lily
Chromosome banding
Cytotaxonomy
Flow cytometry
Genome size
Molecular cytogenetics
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| Resumo: |
ABSTRACT Agapanthus (Agapanthaceae) has 10 species described. However, most taxonomists differ respect to this number because the great phenotypic plasticity of the species. The cytogenetic has been an important tool to aid the plant taxon identification, and to date, all taxa of Agapanthus L'Héritier studied cytologically, presented 2n = 30. Although the species possess large chromosomes, the group is karyologically little explored. This work aimed to increase the cytogenetic knowledge of Agapanthus africanus (L.) Hoffmanns by utilization of chromosome banding techniques with DAPI / CMA3 and Fluorescent in situ Hybridization (FISH). In addition, flow cytometry was used for determination of DNA content and the percentage of AT / GC nitrogenous bases. Plants studied showed 2n = 30 chromosomes, ranging from 4.34 - 8.55 µm, with the karyotype formulae (KF) = 10m + 5sm. Through FISH, one 45S rDNA signal was observed proximally to centromere of the chromosome 7, while for 5S rDNA sites we observed one signal proximally to centromere of chromosome 9. The 2C DNA content estimated for the species was 2C = 24.4 with 59% of AT and 41% of GC. Our data allowed important upgrade for biology and cytotaxonomy of Agapanthus africanus (L.) Hoffmanns.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501727
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| Editor: |
Academia Brasileira de Ciências
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| Relação: |
10.1590/0001-3765201620150514
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| Formato: |
text/html
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| Fonte: |
Anais da Academia Brasileira de Ciências v.88 n.3 suppl.0 2016
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| Direitos: |
info:eu-repo/semantics/openAccess
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