Registro completo |
Provedor de dados: |
Arq. Bras. Med. Vet. Zootec.
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País: |
Brazil
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Título: |
Feline immunodeficiency virusinfection: a comparative study of different diagnostic techniques
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Autores: |
Mortola,E.
Oliva,G.
Risso,M.
Pecoraro,M.
Venturini,M.C.
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Data: |
2004-02-01
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Ano: |
2004
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Palavras-chave: |
Feline immunodeficiency virus
Immunofluorescence assay
Western blot
Polymerase chain reaction
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Resumo: |
This study evaluated an indirect immunofluorescence assay (IFA) to detect feline immunodeficiency virus infection (FIV) antibody in a comprehensive epidemiological survey of FIV in Argentina. IFA modified in our laboratory, was compared with two other immunoassays, western blot (WB) and a sandwich immunochromatographic commercial kit (SI), and also with a direct polymerase chain reaction (PCR) method that detects proviral DNA. IFA showed to be a test with high sensitivity and specificity, and could be useful as a diagnostic tool in epidemiological studies. The presence of a low percentage of results with non-specific reactivity in the IFA could be resolved with further testing or use of an alternative method.
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Tipo: |
Info:eu-repo/semantics/article
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Idioma: |
Inglês
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Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000100003
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Editor: |
Universidade Federal de Minas Gerais, Escola de Veterinária
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Relação: |
10.1590/S0102-09352004000100003
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Formato: |
text/html
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Fonte: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.56 n.1 2004
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Direitos: |
info:eu-repo/semantics/openAccess
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