| Registro completo |
| Provedor de dados: |
Biocell
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| País: |
Argentina
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| Título: |
A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation
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| Autores: |
Capani,Francisco
Saraceno,Ezequiel
Boti,Valeria Romina
Aon-Bertolino,Laura
Fernández,Juan Carlos
Gato,Fernando
Krause,Maria Sol
Giraldez,Lisandro
Ellisman,Mark H.
Coirini,Héctor
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| Data: |
2008-04-01
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| Ano: |
2008
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| Palavras-chave: |
F-actin
Phalloidin-eosin phootoxidation
3-D reconstructions
Electron tomography
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| Resumo: |
Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining and three-dimensional reconstructions provide a valuable tool for revealing aspects of the synaptic morphology that are not available when conventional electron microscopy is used. By applying this selective staining technique and three-dimensional imaging, we uncovered the structural organization of actin in the postsynaptic densities in physiological and pathological conditions.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000100001
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| Editor: |
Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)
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| Formato: |
text/html
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| Fonte: |
Biocell v.32 n.1 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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