| Registro completo |
| Provedor de dados: |
Biocell
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| País: |
Argentina
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| Título: |
Pollen viability of Polygala paniculata L. (Polygalaceae) using different staining methods
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| Autores: |
Frescura,Viviane Dal-Souto
Laughinghouse IV,Haywood Dail
Dorow,Thais Scotti do Canto-
Tedesco,Solange Bosio
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| Data: |
2012-12-01
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| Ano: |
2012
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| Palavras-chave: |
Pollen grains
Barba-de-bode
Acetic orcein
Acetic carmine
Alexander's stain
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| Resumo: |
Polygala paniculata L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as 'barba-de-São-João', 'barba-de-bode', 'vassourinha branca', and 'mimosa'. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander's stain. The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol: acetic acid (3:1) for 24 hours, then stored in ethanol 70% under refrigeration. Six slides per plant, two for each stain, were prepared by squashing, and 300 pollen grains per slide were analyzed. Pollen viability was high (>70%) for most accessions of P. paniculata using the Alexander's stain, which proved the most adequate method to estimate pollen viability.
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| Tipo: |
Info:eu-repo/semantics/report
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300007
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| Editor: |
Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)
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| Formato: |
text/html
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| Fonte: |
Biocell v.36 n.3 2012
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| Direitos: |
info:eu-repo/semantics/openAccess
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