| Registro completo |
| Provedor de dados: |
Biocell
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| País: |
Argentina
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| Título: |
Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells
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| Autores: |
R odriguez,Andrea P.
Tsujigiwa,Hidetsugu
Gunduz,Mehmet
Cengiz,Beyhan
Nagai,Noriyuki
Tamamura,Ryo
Borkosky,Silvia S.
Takagi,Tohru
Inoue,Miho
Nagatsuka,Hitoshi
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| Data: |
2009-04-01
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| Ano: |
2009
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| Palavras-chave: |
MDPC-23 cells
KUSA/A1 cells
Diffusion chamber
RT-PCR analysis
Immunohistochemical analysis
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| Resumo: |
Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin formation. This study shows that MDPC-23 cells are especial cells, which possess morphological and functional characteristics of odontoblast-like cells expressing dentin sialophosphoprotein in vivo. In contrast, dentin sialophosphoprotein gene and protein expression was not detected in both cell lines in vitro. The intra-abdominal diffusion chamber appears as an interesting experimental model for studying phenotypic expression of dental pulp cells in vivo.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100005
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| Editor: |
Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)
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| Formato: |
text/html
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| Fonte: |
Biocell v.33 n.1 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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