Registro completo |
Provedor de dados: |
Biocell
|
País: |
Argentina
|
Título: |
Plant regeneration after long term callus culture in clones of Asparagus officinalis L.
|
Autores: |
Pontaroli,A. C.
Camadro,E. L.
|
Data: |
2005-12-01
|
Ano: |
2005
|
Palavras-chave: |
Callus
Shoot primordia induction
Rooting
Medium x genotype interactions
|
Resumo: |
Callus growth and plant regeneration from long-term callus cultures were studied in two elite clones of Asparagus officinalis cv. Argenteuil, to establish a suitable protocol for a prospective in vitro selection program. Callus initiation and growth was evaluated on MS medium with 3% sucrose, 0.9% agar, 1 mg.l-1 kinetin, and three levels of 2,4-D. The highest callus relative growth was obtained on medium with 1.5 mg.l-1 2,4-D and 1 mg.l-1 kinetin. Shoot primordia (SP) induction from >18-months-old calluses was evaluated on several media; the highest percentage of SP induction (89%) and average number of SP per callus (8.6) were obtained with clone ‘265' on MS medium with 5 mg.l-1 2iP, 1 mg.l-1 IAA, 3% sucrose and 0.9% agar. The highest percentage of root induction (100%) was achieved with clone ‘265' on MS medium with 0.1 mg.l-1 kinetin, 0.1 mg.l-1 NAA, 1.32 mg.l-1 ancymidol, 7% glucose and 0.8% agar. Important medium x genotype interactions were detected, pointing to the need of adjusting this and other in vitro protocols for specific asparagus genotypes.
|
Tipo: |
Info:eu-repo/semantics/other
|
Idioma: |
Inglês
|
Identificador: |
http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300009
|
Editor: |
Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)
|
Formato: |
text/html
|
Fonte: |
Biocell v.29 n.3 2005
|
Direitos: |
info:eu-repo/semantics/openAccess
|
|