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Provedor de dados:  48
País:  Chile
Título:  High-level expression of modified gene encoding human adiponectin in transgenic rice
Autores:  Liu,Feng
Zhao,Yi-Ying
Zheng,Jin-Gui
Data:  2011-01-01
Ano:  2011
Palavras-chave:  Human adiponectin
Transgenic rice
Modified gene
Anti-diabetic
Resumo:  Adiponectin is a polypeptide specifically secreted from human adipocytes, and its deficiency is closely linked to increased obesity and type II diabetes. There is an urgent demand for large-scale production of human adiponectin for pharmaceutical applications. Here, we report that we have successfully obtained a high-level of expression of modified genes encoding human adiponectin in transgenic rice. The 735 bp cDNA of the native human sequence was adopted to rice codon usage, fused to the translation initiation sequence in the N terminus and to the KDEL signal sequence in the C terminus. An amplification promoting sequence acting as an enhancer of transcription was also introduced to enhance gene expression. The presence of the transgene and mRNA transcripts was confirmed by PCR, Southern blot and RT-PCR. Western blot analysis revealed that a protein of approximately 30 kDa was produced in rice leaves. ELISA analysis was used to determine the amount of recombinant adiponectin in transformants with the modified gene in up to 0.32% of total soluble leaf protein. Our results establish the feasibility of high-level expression of recombinant human adiponectin in transgenic rice.
Tipo:  Journal article
Idioma:  Inglês
Identificador:  http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400009
Editor:  Sociedad de Biología de Chile
Formato:  text/html
Fonte:  Biological Research v.44 n.4 2011
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