| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
pH controls spermatozoa motility in the Pacific oyster ( Crassostrea gigas )
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| Autores: |
Boulais, Myrina
Suquet, Marc
Arsenault-pernet, Marie Eve Julie
Malo, Florent
Queau, Isabelle
Pignet, Patricia
Ratiskol, Dominique
Le Grand, Jacqueline
Huber, Matthias
Cosson, Jacky
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| Data: |
2018-03
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| Ano: |
2018
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| Palavras-chave: |
Seminal fluid
Spermatozoa
Motility
PH
Salinity
Ions
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| Resumo: |
Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster. The acidic pH of the gonad (5.82 ± 0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH4Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared to NH4Cl-free SW, revealing the role of pHi in triggering sperm movement. Percentage of motile spermatozoa activated in Na+-free artificial seawater (ASW) was highly reduced compared to ASW, suggesting that change of pHi triggering sperm motility was mediated by a Na+/H+ exchanger. Motility and swimming speed were highest in salinities between 33.8 and 42.7‰ (within a range of 0 to 50 ‰), and pH values above 7.5 (within a range of 4.5 to 9.5).
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00428/53976/55137.pdf
DOI:10.1242/bio.031427
https://archimer.ifremer.fr/doc/00428/53976/
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| Editor: |
Company Of Biologists Ltd
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| Formato: |
application/pdf
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| Fonte: |
Biology Open (2046-6390) (Company Of Biologists Ltd), 2018-03 , Vol. 7 , N. 3 , P. bio031427 (8p.)
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| Direitos: |
2018. Published by The Company of Biologists Ltd
info:eu-repo/semantics/openAccess
restricted use
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