| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Potential of fascaplysin and palauolide from Fascaplysinopsis cf reticulata to reduce the risk of bacterial infection in fish farming
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| Autores: |
Mai, Tepoerau
Toullec, Jordan
Van Wynsberge, Simon
Besson, Marc
Soulet, Stephanie
Petek, Sylvain
Aliotti, Emmanuelle
Ekins, Merrick
Hall, Kathryn
Erpenbeck, Dirk
Lecchini, David
Beniddir, Mehdi A.
Saulnier, Denis
Debitus, Cecile
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| Data: |
2019-12
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| Ano: |
2019
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| Palavras-chave: |
Porifera
Marine natural products
Quorum sensing inhibitors
Antibiotic
Fascaplysinopsis cf reticulata
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| Resumo: |
Marine natural products isolated from the sponge Fascaplysinopsis cf reticulata, in French Polynesia, were investigated as an alternative to antibiotics to control pathogens in aquaculture. The overuse of antibiotics in aquaculture is largely considered to be an environmental pollution, because it supports the transfer of antibiotic resistance genes within the aquatic environment. One environmentally friendly alternative to antibiotics is the use of quorum sensing inhibitors (QSIs). Quorum sensing (QS) is a regulatory mechanism in bacteria which control virulence factors through the secretion of autoinducers (AIs), such as acyl-homoserine lactone (AHL) in gram-negative bacteria. Vibrio harveyi QS is controlled through three parallel pathways: HAI-1, AI-2, and CAI-1. Bioassay-guided purification of F. cf reticulata extract was conducted on two bacterial species, i.e., Tenacibaculum maritimum and V. harveyi for antibiotic and QS inhibition bioactivities. Toxicity bioassay of fractions was also evaluated on the freshwater fish Poecilia reticulata and the marine fish Acanthurus triostegus. Cyclohexanic and dichloromethane fractions of F. cf reticulata exhibited QS inhibition on V. harveyi and antibiotic bioactivities on V. harveyi and T. maritimum, respectively. Palauolide (1) and fascaplysin (2) were purified as major molecules from the cyclohexanic and dichloromethane fractions, respectively. Palauolide inhibited QS of V. harveyi through HAI-1 QS pathway at 50 μg ml–1 (26 μM), while fascaplysin affected the bacterial growth of V. harveyi (50 μg ml–1) and T. maritimum (0.25 μg). The toxicity of fascaplysin-enriched fraction (FEF) was evaluated and exhibited a toxic effect against fish at 50 μg ml–1. This study demonstrated for the first time the QSI potential of palauolide (1). Future research may assess the toxicity of both the cyclohexanic fraction of the sponge and palauolide (1) on fish, to confirm their potential as alternative to antibiotics in fish farming.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00598/71052/69366.pdf
DOI:10.1186/s41240-019-0145-0
https://archimer.ifremer.fr/doc/00598/71052/
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| Editor: |
Springer Science and Business Media LLC
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| Formato: |
application/pdf
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| Fonte: |
Fisheries and Aquatic Sciences (2234-1757) (Springer Science and Business Media LLC), 2019-12 , Vol. 22 , N. 1 , P. 30 (11p.)
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| Direitos: |
info:eu-repo/semantics/openAccess
restricted use
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