| Registro completo |
| Provedor de dados: |
ArchiMer
|
| País: |
France
|
| Título: |
Expressed sequence tags from heat-shocked seagrass Zostera noltii (Hornemann) from its southern distribution range
|
| Autores: |
Massa, Sonia I.
Pearson, Gareth A.
Aires, Tania
Kube, Michael
Olsen, Jeanine L.
Reinhardt, Richard
Serrao, Ester A.
Arnaud Haond, Sophie
|
| Data: |
2011-09
|
| Ano: |
2011
|
| Palavras-chave: |
EST library
High temperature stress
Abiotic stress response
Climate change
|
| Resumo: |
Predicted global climate change threatens the distributional ranges of species worldwide. We identified genes expressed in the intertidal seagrass Zostera midi during recovery from a simulated low tide heat-shock exposure. Five Expressed Sequence Tag (EST) libraries were compared, corresponding to four recovery times following sub-lethal temperature stress, and a non-stressed control. We sequenced and analyzed 7009 sequence reads from 30 min, 2 h, 4 h and 24 h after the beginning of the heat-shock (AHS), and 1585 from the control library, for a total of 8594 sequence reads. Among 51 Tentative UniGenes (TUGs) exhibiting significantly different expression between libraries, 19 (37.3%) were identified as 'molecular chaperones' and were over-expressed following heat-shock, while 12 (23.5%) were 'photosynthesis TUGs' generally under-expressed in heat-shocked plants. A time course analysis of expression showed a rapid increase in expression of the molecular chaperone class, most of which were heat-shock proteins; which increased from 2 sequence reads in the control library to almost 230 in the 30 min AHS library, followed by a slow decrease during further recovery. In contrast, 'photosynthesis TUGs' were under-expressed 30 min AHS compared with the control library, and declined progressively with recovery time in the stress libraries, with a total of 29 sequence reads 24 h AHS, compared with 125 in the control. A total of 4734 TUGs were screened for EST-Single Sequence Repeats (EST-SSRs) and 86 microsatellites were identified. (C) 2011 Elsevier B.V. All rights reserved.
|
| Tipo: |
Text
|
| Idioma: |
Inglês
|
| Identificador: |
http://archimer.ifremer.fr/doc/00044/15559/13326.pdf
DOI:10.1016/j.margen.2011.04.003
|
| Editor: |
Elsevier Science Bv
|
| Relação: |
http://archimer.ifremer.fr/doc/00044/15559/
|
| Formato: |
application/pdf
|
| Fonte: |
Marine Genomics (1874-7787) (Elsevier Science Bv), 2011-09 , Vol. 4 , N. 3 , P. 181-188
|
| Direitos: |
2011 Elsevier B.V. All rights reserved.
|
|
