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Provedor de dados:  ArchiMer
País:  France
Título:  Accumulation curves of environmental DNA sequences predict coastal fish diversity in the coral triangle
Autores:  Juhel, Jean-baptiste
Utama, Rizkie S.
Marques, Virginie
Vimono, Indra B.
Sugeha, Hagi Yulia
Kadarusman,
Pouyaud, Laurent
Dejean, Tony
Mouillot, David
Hocdé, Régis
Data:  2020-07
Ano:  2020
Palavras-chave:  EDNA metabarcoding
Sequence clustering
Operational Taxonomic Unit
Diversity assessment
Detectability
Resumo:  Environmental DNA (eDNA) has the potential to provide more comprehensive biodiversity assessments, particularly for vertebrates in species-rich regions. However, this method requires the completeness of a reference database (i.e. a list of DNA sequences attached to each species), which is not currently achieved for many taxa and ecosystems. As an alternative, a range of operational taxonomic units (OTUs) can be extracted from eDNA metabarcoding. However, the extent to which the diversity of OTUs provided by a limited eDNA sampling effort can predict regional species diversity is unknown. Here, by modelling OTU accumulation curves of eDNA seawater samples across the Coral Triangle, we obtained an asymptote reaching 1531 fish OTUs, while 1611 fish species are recorded in the region. We also accurately predict (R² = 0.92) the distribution of species richness among fish families from OTU-based asymptotes. Thus, the multi-model framework of OTU accumulation curves extends the use of eDNA metabarcoding in ecology, biogeography and conservation.
Tipo:  Text
Idioma:  Inglês
Identificador:  https://archimer.ifremer.fr/doc/00640/75232/79601.pdf

DOI:10.1098/rspb.2020.0248

https://archimer.ifremer.fr/doc/00640/75232/
Editor:  The Royal Society
Formato:  application/pdf
Fonte:  Proceedings Of The Royal Society B-biological Sciences (0962-8452) (The Royal Society), 2020-07 , Vol. 287 , N. 1930 , P. 20200248 (10p.)
Direitos:  info:eu-repo/semantics/openAccess

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