| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Optimization of growth and bacteriocin activity of the food bioprotective Carnobacterium divergens V41 in an animal origin protein free medium
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| Autores: |
Brillet-viel, Anne
Pilet, Marie-france
Courcoux, Philippe
Prevost, Hervé
Leroi, Francoise
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| Data: |
2016-08
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| Ano: |
2016
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| Palavras-chave: |
Optimization
Carnobacterium
Bacteriocin
Experimental design
Bioreactor
Growth
Cold-smoked salmon
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| Resumo: |
Optimization of Carnobacterium divergens V41 growth and bacteriocin activity in a culture medium deprived of animal protein, needs for food bioprotection, was performed by using a statistical approach. In a screening experiment, twelve factors (pH, temperature, carbohydrates, NaCl, yeast extract, soy peptone, sodium acetate, ammonium citrate, magnesium sulphate, manganese sulphate, ascorbic acid and thiamine) were tested for their influence on the maximal growth and bacteriocin activity using a two-level incomplete factorial design with 192 experiments performed in microtiter plate wells. Based on results, a basic medium was developed and three variables (pH, temperature and carbohydrates concentration) were selected for a scale-up study in bioreactor. A 23 complete factorial design was performed, allowing the estimation of linear effects of factors and all the first order interactions. The best conditions for the cell production were obtained with a temperature of 15°C and a carbohydrates concentration of 20 g/l whatever the pH (in the range 6.5-8), and the best conditions for bacteriocin activity were obtained at 15°C and pH 6.5 whatever the carbohydrates concentration (in the range 2-20 g/l). The predicted final count of C. divergens V41 and the bacteriocin activity under the optimized conditions (15°C, pH 6.5, 20 g/l carbohydrates) were 2.4 x 1010 CFU/ml and 819200 AU/ml respectively. C. divergens V41 cells cultivated in the optimized conditions were able to grow in cold-smoked salmon and totally inhibited the growth of Listeria monocytogenes (< 50 CFU g-1) during five weeks of vacuum storage at 4° and 8°C.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00345/45615/45236.pdf
DOI:10.3389/fmars.2016.00128
https://archimer.ifremer.fr/doc/00345/45615/
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| Editor: |
Frontiers Media S.A
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| Formato: |
application/pdf
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| Fonte: |
Frontiers in Marine Science (2296-7745) (Frontiers Media S.A), 2016-08 , Vol. 3 , N. 128 , P. 13p.
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| Direitos: |
2016 Brillet-viel, Pilet, Courcoux, Prévost and Leroi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
info:eu-repo/semantics/openAccess
restricted use
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