Registro completo |
Provedor de dados: |
ArchiMer
|
País: |
France
|
Título: |
A Functional Study of Transforming Growth Factor-Beta from the Gonad of Pacific Oyster Crassostrea gigas
|
Autores: |
Corporeau, Charlotte
Groisillier, Agnes
Jeudy, Alexandra
Barbeyron, Tristan
Fleury, Elodie
Fabioux, Caroline
Czjzek, Mirjam
Huvet, Arnaud
|
Data: |
2011-10
|
Ano: |
2011
|
Palavras-chave: |
Transforming growth factor-beta
Bacterial expression
In vivo antibody inhibition
Reproduction
Crassostrea gigas
Marine bivalve
|
Resumo: |
The transforming growth factor (TGF)-beta superfamily is a group of important growth factors involved in multiple processes such as differentiation, cell proliferation, apoptosis and cellular growth. In the Pacific oyster Crassostrea gigas, the oyster gonadal (og) TGF-beta gene was recently characterized through genome-wide expression profiling of oyster lines selected to be resistant or susceptible to summer mortality. Og TGF-beta appeared specifically expressed in the gonad to reach a maximum when gonads are fully mature, which singularly contrasts with the pleiotropic roles commonly ascribed to most TGF-beta family members. The function of og TGF-beta protein in oysters is unknown, and defining its role remains challenging. In this study, we develop a rapid bacterial production system to obtain recombinant og TGF-beta protein, and we demonstrate that og TGF-beta is processed by furin to a mature form of the protein. This mature form can be detected in vivo in the gonad. Functional inhibition of mature og TGF-beta in the gonad was conducted by inactivation of the protein using injection of antibodies. We show that inhibition of og TGF-beta function tends to reduce gonadic area. We conclude that mature og TGF-beta probably functions as an activator of germ cells development in oyster.
|
Tipo: |
Text
|
Idioma: |
Inglês
|
Identificador: |
http://archimer.ifremer.fr/doc/00042/15371/13438.pdf
DOI:10.1007/s10126-010-9361-4
|
Editor: |
Springer
|
Relação: |
http://archimer.ifremer.fr/doc/00042/15371/
|
Formato: |
application/pdf
|
Fonte: |
Marine Biotechnology (1436-2228) (Springer), 2011-10 , Vol. 13 , N. 5 , P. 971-980
|
Direitos: |
Springer Science+Business Media, LLC 2011
|
|