| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
The development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of abalone herpesvirus DNA
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| Autores: |
Chen, Meilin
Kuo, S. T.
Renault, Tristan
Chang, P. H.
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| Data: |
2014-02
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| Ano: |
2014
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| Palavras-chave: |
Herpesvirus
Abalone
Haliotis diversicolor supertexta
Polymerase chain reaction
Loop-mediated isothermal amplification
SYBR green PCR
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| Resumo: |
A loop-mediated isothermal amplification (LAMP) assay was developed for the detection of abalone herpesvirus DNA. Two pairs of primers were designed, based on the sequence of the DNA polymerase gene of abalone herpesvirus. The reaction temperature and time were optimized to 63 °C and 60 min, respectively. LAMP amplicons were analyzed by 2% agarose gel electrophoresis or by visual inspection of a colour change emitted by fluorescent dye. The method developed was specific for the detection of abalone herpesvirus, without cross-reactions with other tested herpesviruses including ostreid herpesvirus 1 (OsHV-1), European eel herpesvirus, koi herpesvirus (KHV) and an avian herpesvirus. The LAMP assay was 100 folds more sensitive than a conventional PCR and 10 folds less sensitive than a SYBR Green PCR. These results indicate that the developed LAMP assay is a simple, rapid, sensitive, specific and reliable technique for the detection of abalone herpesvirus.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00166/27690/25882.pdf
DOI:10.1016/j.jviromet.2013.11.011
https://archimer.ifremer.fr/doc/00166/27690/
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| Editor: |
Elsevier Science Bv
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| Formato: |
application/pdf
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| Fonte: |
Journal Of Virological Methods (0166-0934) (Elsevier Science Bv), 2014-02 , Vol. 197 , P. 199-203
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| Direitos: |
This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
info:eu-repo/semantics/openAccess
restricted use
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