| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Identification and separation of saxitoxins using hydrophilic interaction liquid chromatography coupled to traveling wave ion mobility-mass spectrometry
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| Autores: |
Poyer, Salome
Loutelier-bourhis, Corinne
Coadou, Gael
Mondeguer, Florence
Enche, Julien
Bossee, Anne
Hess, Philipp
Afonso, Carlos
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| Data: |
2015-01
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| Ano: |
2015
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| Palavras-chave: |
Saxitoxin
Ion mobility-mass spectrometry
Isomers
Multidimensional separation
HILIC
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| Resumo: |
The aim of this work was to develop a reliable and efficient analytical method to characterise and differentiate saxitoxin analogues (STX), including sulphated (gonyautoxins, GTX) and non-sulphated analogues. For this purpose, hydrophilic interaction liquid chromatography (HILIC) was used to separate sulphated analogues. We also resorted to ion mobility spectrometry to differentiate the STX analogues because this technique adds a new dimension of separation based on ion gas phase conformation. Positive and negative ionisation modes were used for gonyautoxins while positive ionisation mode was used for non-sulphated analogues. Subsequently, the coupling of these three complementary techniques, HILIC-IM-MS, permitted the separation and identification of STX analogues; isomer differentiation was achieved in HILIC dimension while non-sulphated analogues were separated in the IM-MS dimension. Additional structural characteristics concerning the conformation of STXs could be obtained using IM-MS measurements. Thus, the collision cross sections (CCS) of STXs are reported for the first time in the positive ionisation mode. These experimental CCSs correlated well with the calculated CCS values using the trajectory method.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00249/36067/34924.pdf
DOI:10.1002/jms.3515
https://archimer.ifremer.fr/doc/00249/36067/
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| Editor: |
Wiley-blackwell
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| Formato: |
application/pdf
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| Fonte: |
Journal Of Mass Spectrometry (1076-5174) (Wiley-blackwell), 2015-01 , Vol. 50 , N. 1 , P. 175-181
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| Direitos: |
2015 John Wiley & Sons, Ltd.
info:eu-repo/semantics/openAccess
restricted use
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