| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi
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| Autores: |
Fisher, Matthew C.
Ghosh, Pria
Shelton, Jennifer M. G.
Bates, Kieran
Brookes, Lola
Wierzbicki, Claudia
Rosa, Goncalo M.
Farrer, Rhys A.
Aanensen, David M.
Alvarado-rybak, Mario
Bataille, Arnaud
Berger, Lee
Boell, Susanne
Bosch, Jaime
Clare, Frances C.
Courtois, Elodie A.
Crottini, Angelica
Cunningham, Andrew A.
Doherty-bone, Thomas M.
Gebresenbet, Fikirte
Gower, David J.
Hoglund, Jacob
James, Timothy Y
Jenkinson, Thomas S.
Kosch, Tiffany A.
Lambertini, Carolina
Laurila, Anssi
Lin, Chun-fu
Loyau, Adeline
Martel, An
Meurling, Sara
Miaud, Claude
Minting, Pete
Ndriantsoa, Serge
O'Hanlon, Simon J.
Pasmans, Frank
Rakotonanahary, Tsanta
Rabemananjara, Falitiana C. E.
Ribeiro, Luisa P.
Schmeller, Dirk S.
Schmidt, Benedikt R.
Skerratt, Lee
Smith, Freya
Soto-azat, Claudio
Tessa, Giulia
Toledo, Luis Felipe
Valenzuela-sanchez, Andres
Verster, Ruhan
Voeroes, Judit
Waldman, Bruce
Webb, Rebecca J.
Weldon, Che
Wombwell, Emma
Zamudio, Kelly R.
Longcore, Joyce E.
Garner, Trenton W. J.
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| Data: |
2018-05
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| Ano: |
2018
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| Resumo: |
Parasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and biopsies from toe webbing rather than euthanizing hosts, and distributed the protocol to researchers as part of the BiodivERsA project RACE; here called the RML protocol. In tandem, we developed a lethal procedure for isolating chytrids from tadpole mouthparts. Reviewing a database of use a decade after their inception, we find that these methods have been applied across 5 continents, 23 countries and in 62 amphibian species. Isolation of chytrids by the non-lethal RML protocol occured in 18% of attempts with 207 fungal isolates and three species of chytrid being recovered. Isolation of chytrids from tadpoles occured in 43% of attempts with 334 fungal isolates of one species (Batrachochytrium dendrobatidis) being recovered. Together, these methods have resulted in a significant reduction and refinement of our use of threatened amphibian species and have improved our ability to work with this group of emerging pathogens.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00442/55317/78944.pdf
https://archimer.ifremer.fr/doc/00442/55317/78945.pdf
https://archimer.ifremer.fr/doc/00442/55317/78946.pdf
DOI:10.1038/s41598-018-24472-2
https://archimer.ifremer.fr/doc/00442/55317/
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| Editor: |
Nature Publishing Group
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| Formato: |
application/pdf
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| Fonte: |
Scientific Reports (2045-2322) (Nature Publishing Group), 2018-05 , Vol. 8 , P. 7772 (8p.)
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| Direitos: |
info:eu-repo/semantics/openAccess
restricted use
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