| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
High-Throughput Identification of Candidate Strains for Biopreservation by Using Bioluminescent Listeria monocytogenes
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| Autores: |
El Kheir, Sara M.
Cherrat, Lamia
Awussi, Ahoefa A.
Ramia, Nancy E.
Taha, Samir
Rahman, Abdur
Passerini, Delphine
Leroi, Francoise
Petit, Jeremy
Mangavel, Cecile
Revol-junelles, Anne-marie
Borges, Frederic
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| Data: |
2018-08
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| Ano: |
2018
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| Palavras-chave: |
Biopreservation
Listeria monocytogenes
High-throughput screening assays
Bioluminescence
Competition
Mixed culture
Bioprotection
Antibacterial activities
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| Resumo: |
This article describes a method for high-throughput competition assays using a bioluminescent strain of L. monocytogenes. This method is based on the use of the luminescent indicator strain L. monocytogenes EGDelux. The luminescence of this strain is correlated to growth, which make it suitable to monitor the growth of L. monocytogenes in mixed cultures. To this aim, luminescence kinetics were converted into a single numerical value, called the Luminescence Disturbance Indicator (LDI), which takes into account growth inhibition phenomena resulting in latency increase, decrease in the luminescence rate, or reduction of the maximum luminescence. The LDI allows to automatically and simultaneously handle multiple competition assays which are required for high-throughput screening (HTS) approaches. The method was applied to screen a collection of 1810 strains isolated from raw cow’s milk in order to identify non-acidifying strains with anti-L. monocytogenes bioprotection properties. This method was also successfully used to identify anti-L. monocytogenes candidates within a collection of Lactococcus piscium, a species where antagonism was previously described as non-diffusible and requiring cell-to-cell contact. In conclusion, bioluminescent L. monocytogenes can be used in HTS to identify strains with anti-L. monocytogenes bioprotection properties, irrespectively of the inhibition mechanism.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00454/56534/58236.pdf
https://archimer.ifremer.fr/doc/00454/56534/58237.xlsx
DOI:10.3389/fmicb.2018.01883
https://archimer.ifremer.fr/doc/00454/56534/
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| Editor: |
Frontiers Media Sa
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| Formato: |
application/pdf
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| Fonte: |
Frontiers In Microbiology (1664-302X) (Frontiers Media Sa), 2018-08 , Vol. 9 , N. 1883 , P. 10p.
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| Direitos: |
2018 El Kheir, Cherrat, Awussi, Ramia, Taha, Rahman, Passerini, Leroi, Petit, Mangavel, Revol-Junelles and Borges. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with acce
info:eu-repo/semantics/openAccess
restricted use
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