| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Oligonucleotide quartz crystal microbalance sensor for the microalgae Alexandrium minutum (Dinophyceae)
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| Autores: |
Lazerges, M
Perrot, H
Antoine, Elisabeth
Defontaine, Audrey
Compere, Chantal
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| Data: |
2006
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| Ano: |
2006
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| Palavras-chave: |
DNA
Alexandrium minutum
Microbalance
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| Resumo: |
We report the immobilization on a gold surface of a 20-base DNA probe labeled with disulfide group and on the selective hybridization with the complementary 20-base DNA strand. The oligonucleotide probe is the complementary strand of a partial sequence of the gene encoding for a large ribosomal RNA sub-unit which is a coding sequence of Alexandrium minutum DNA, a microalgae that produces neurotoxins responsible for paralytic shellfish poisoning on European and Asian coasts. The kinetics of DNA probe immobilization and hybridization were monitored in situ by using a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the setup is stable to within a few hertz, corresponding to the nanogram, scale, for 3 h and makes it possible to follow frequency change from immobilization of the probe to hybridization of the complementary DNA target. This setup constitutes a biosensor, which is sensitive and selective, and the hybridization ratio between hybridized complementary DNA and immobilized DNA probes is 47%.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2006/publication-1354.pdf
DOI:10.1016/j.bios.2005.05.013
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| Editor: |
Elsevier
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| Relação: |
http://archimer.ifremer.fr/doc/00000/1354/
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| Formato: |
application/pdf
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| Fonte: |
Biosensors and Bioelectronics (0956-5663) (Elsevier), 2006 , Vol. 21 , N. 7 , P. 1355-1358
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| Direitos: |
2005 Elsevier B.V. All rights reserved
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