| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Subcellular distribution of Ag, Cd, Co, Cu, Fe, Mn, Ph, and Zn in the digestive gland of the common cuttlefish Sepia officinalis
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| Autores: |
Bustamante, Paco
Bertrand, M
Boucaud Camou, E
Miramand, Pierre
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| Data: |
2006-12
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| Ano: |
2006
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| Palavras-chave: |
Sepia officinalis
Chromatography
Metallothionein
Detoxification
Trace element
Metal
Cephalopod
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| Resumo: |
The subcellular fractionation of the digestive gland cells of the common cuttlefish Sepia officinalis was performed to investigate the distribution of metals between organelles and cytosol and the different cytosolic fractions separated by gel-filtration chromatography. Total metal concentrations vary over 3 orders of magnitude, ranging from dwt for Pb to dwt for Zn. With the exception of Cd, Co, and Cu, metals were mostly bound to the organelles. Whereas no specific organelle compartment was found for Mn, Ph, and Zn, Fe was mainly associated with nucleus, brown body, and "boule" fraction (i.e., 52%) and 44% of the total Ag was contained in the lysosomal and mitochondria enriched fraction. The link of metals with hydrosoluble proteins in the cytosolic fraction was investigated at 254 and 280 not. Direct relationship between cytosolic metal and metallothioneins could only be established for Ag and Cu, whereas Cd and Zn seem to mainly bind high (> 70 kDa) and low (< 4 kDa) molecular weight proteins.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2006/publication-2532.pdf
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| Editor: |
National Shellfisheries Association
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| Relação: |
http://archimer.ifremer.fr/doc/00000/2532/
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| Formato: |
application/pdf
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| Fonte: |
The Journal of Shellfish Research (0730-8000) (National Shellfisheries Association), 2006-12 , Vol. 25 , N. 3 , P. 987-993
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| Direitos: |
2006 NSA
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