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Provedor de dados:  ArchiMer
País:  France
Título:  Enhancement of domoic acid neurotoxicity on Diptera larvae bioassay by marine fungal metabolites
Autores:  Ruiz, Nicolas
Petit, Karina
Vansteelandt, Marieke
Kerzaon, Isabelle
Baudet, Joseph
Amzil, Zouher
Biard, Jean-francois
Grovel, Olivier
Pouchus, Yves Francois
Data:  2010-04
Ano:  2010
Palavras-chave:  Domoic acid
Marine-derived fungal metabolite
Diptera larvae bioassay
Resumo:  Peptaibols are small linear fungal peptides which are produced in the marine environment. They exhibit neurotoxicity by forming pores in neuronal membranes. This work describes their combine effect with domoic acid, a neurotoxic phycotoxin, on Diptera larvae. The Acute toxicity bioassay on this biological model was tested with a panel of different toxins (microbial, algal or fungal). It allowed the discrimination of neurotoxins and non-neurotoxic toxins, and an evaluation of the toxicity level (MED and ED50) which were correlated with published LD50 in mice for neurotoxins tested. The highest activities on this test were found for Na+ channel blockers tetrodotoxin (ED50 = 0.026 mg/kg) and saxitoxin (ED50 = 0.18 mg/kg). Domoic acid was less active with an ED50 = 7.6 mg/kg. For synergism study, longibrachin-A-I, a 20-mer peptaibol isolated from cultures of a marine-derived strain of Trichoderma longibrachiatum Rifai was chosen. Bioassay results confirmed its neuroactivity. Its level of toxicity (ED50 = 270 mg/kg) was lower than those of phycotoxins tested but higher than mycotoxin ones. Injected together, longibrachin-A-I and domoic acid exhibited an increase of their activities. With doses of longibrachin-A-I below its Minimal Effective Dose (MED), the synergism factor which expresses the enhancement of domoic acid toxicity could reach 34.5. Both domoic acid and longibrachin-A-I are acting on ion channels and pores in neuronal membranes which contribute to the intake of Ca2+ into cells.
Tipo:  Text
Idioma:  Inglês

Editor:  Elsevier
Formato:  application/pdf
Fonte:  Toxicon (0041-0101) (Elsevier), 2010-04 , Vol. 55 , N. 4 , P. 805-810
Direitos:  2009 Elsevier Inc. All rights reserved.

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