Registro completo |
Provedor de dados: |
ArchiMer
|
País: |
France
|
Título: |
Functional Screening of Hydrolytic Activities Reveals an Extremely Thermostable Cellulase from a Deep-Sea Archaeon
|
Autores: |
Leis, Benedikt
Heinze, Simon
Angelov, Angel
Pham, Vu Thuy Trang
Thürmer, Andrea
Jebbar, Mohamed
Golyshin, Peter N.
Streit, Wolfgang R.
Daniel, Rolf
Liebl, Wolfgang
|
Data: |
2015-07
|
Ano: |
2015
|
Palavras-chave: |
Functional screenings
Extreme thermostable protein
Archaeal endoglucanase
Enzymatic characterization
|
Resumo: |
Extreme habitats serve as a source of enzymes that are active under extreme conditions and are candidates for industrial applications. In this work, six large-insert mixed genomic libraries were screened for hydrolase activities in a broad temperature range (8–70°C). Among a variety of hydrolytic activities, one fosmid clone, derived from a library of pooled isolates of hyperthermophilic archaea from deep sea vents, displayed hydrolytic activity on carboxymethyl cellulose substrate plates at 70°C but not at lower temperatures. Sequence analysis of the fosmid insert revealed a gene encoding a novel glycoside hydrolase family 12 (GHF12) endo-1,4-β-glucanase, termed Cel12E. The enzyme shares 45% sequence identity with a protein from the archaeon Thermococcus sp. AM4 and displays a unique multidomain architecture. Biochemical characterization of Cel12E revealed a remarkably thermostable protein, which appears to be of archaeal origin. The enzyme displayed maximum activity at 92°C and was active on a variety of linear 1,4-β-glucans like carboxymethyl cellulose, β-glucan, lichenan, and phosphoric acid swollen cellulose. The protein is able to bind to various insoluble β-glucans. Product pattern analysis indicated that Cel12E is an endo-cleaving β-glucanase. Cel12E expands the toolbox of hyperthermostable archaeal cellulases with biotechnological potential.
|
Tipo: |
Text
|
Idioma: |
Inglês
|
Identificador: |
https://archimer.ifremer.fr/doc/00601/71319/69744.pdf
https://archimer.ifremer.fr/doc/00601/71319/69745.pdf
https://archimer.ifremer.fr/doc/00601/71319/69746.pdf
DOI:10.3389/fbioe.2015.00095
https://archimer.ifremer.fr/doc/00601/71319/
|
Editor: |
Frontiers Media SA
|
Relação: |
info:eu-repo/grantAgreement/EC/FP7/287589/EU//MICRO B3
info:eu-repo/grantAgreement/EC/FP7/226977/EU//MAMBA
|
Formato: |
application/pdf
|
Fonte: |
Frontiers in Bioengineering and Biotechnology (2296-4185) (Frontiers Media SA), 2015-07 , Vol. 3 , N. 95 , P. 10p.
|
Direitos: |
info:eu-repo/semantics/openAccess
restricted use
|
|