| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
RpoB-PCR amplified gene and temporal temperature gradient gel electrophoresis: a rapid tool to analyse bacterial strains representative of cold-smoked salmon microflora
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| Autores: |
Giacomazzi, Sophie
Leroi, Francoise
L'Henaff, Cecile
Joffraud, Jean-jacques
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| Data: |
2004-02
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| Ano: |
2004
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| Palavras-chave: |
TTGE
RpoB
Nested PCR
Cold smoked salmon flora
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| Resumo: |
Aims: To evaluate rpoB gene as a biomarker of microbial biodiversity associated to cold-smoked salmon by a novel nested-polymerase chain reaction/temporal temperature gradient gel electrophoresis (PCR/TTGE) technique applied on pure cultures of reference strains. Methods and Results: DNA obtained from pure cultures of reference strains was used in a succession of a first PCR amplification of rpoB fragment with degenerated nonclamped primers and a nested-PCR with nondegenerated clamped primers. PCR products were then applied on a TTGE gel in order to analyse strains profile. High quantity of nested-PCR products were obtained for each tested strain and TTGE profiles showed a good separation between the different reference bacteria and an easy way to associate one band to one species. Conclusion: The nested-PCR/TTGE technique used in this study is a promising way of investigating bacterial community structure of cold-smoked salmon or other food matrix. Significance and Impact of the Study: Because of its single copy state leading to single band profiles in TTGE, rpoB constitute a good potential molecular marker for further development of cold-smoked salmon biodiversity analysis.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2004/publication-579.pdf
DOI:10.1111/j.1472-765X.2003.01463.x
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| Editor: |
Blackwell science
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| Relação: |
http://archimer.ifremer.fr/doc/00000/579/
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| Formato: |
application/pdf
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| Fonte: |
Letters in Applied Microbiology (0266-8254) (Blackwell science), 2004-02 , Vol. 38 , N. 2 , P. 130-134
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| Direitos: |
2004 Blackwell Synergy
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