| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Entrapment of anaerobic thermophilic and hyperthermophilic marine microorganisms in a gellan/xanthan matrix
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| Autores: |
Landreau, M.
Duthoit, Frederique
Claeys-bruno, M.
Vandenabeele-trambouze, O.
Aubry, T.
Godfroy, Anne
Le Blay, Gwenaelle
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| Data: |
2016-06
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| Ano: |
2016
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| Palavras-chave: |
(hyper)thermophilic marine micro-organisms
Anaerobiosis
Entrapment
Gellan
Immobilization
Xanthan
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| Resumo: |
Aims The aims of this study were (i) to develop a protocol for the entrapment of anaerobic (hyper)thermophilic marine micro-organisms; (ii) to test the use of the chosen polymers in a range of physical and chemical conditions and (iii) to validate the method with batch cultures. Methods and Results The best conditions for immobilization were obtained at 80°C with gellan and xanthan gums. After 5-week incubation, beads showed a good resistance to all tested conditions except those simultaneously including high temperature (100°C), low NaCl (<0∙5 mol l−1) and extreme pH (4/8). To confirm the method efficiency, batch cultures with immobilized Thermosipho sp. strain AT1272 and Thermococcus kodakarensis strain KOD1 showed an absence of detrimental effect on cell viability and a good growth within and outside the beads. Conclusion This suggests that entrapment in a gellan–xanthan matrix could be employed for the culture of anaerobic (hyper)thermophilic marine micro-organisms. Significance and Impact of the Study (Hyper)thermophilic marine micro-organisms possess a high biotechnological potential. Generally microbial cells are grown as free-cell cultures. The use of immobilized cells may offer several advantages such as protection against phage attack, high cell biomass and better production rate of desired metabolites.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00332/44352/44000.pdf
DOI:10.1111/jam.13118
http://archimer.ifremer.fr/doc/00332/44352/
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| Editor: |
Wiley-blackwell
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| Relação: |
info:eu-repo/grantAgreement/EC/FP7/311975/EU//MACUMBA
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| Formato: |
application/pdf
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| Fonte: |
Journal Of Applied Microbiology (1364-5072) (Wiley-blackwell), 2016-06 , Vol. 120 , N. 6 , P. 1531-1541
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| Direitos: |
2016 The Society for Applied Microbiology
info:eu-repo/semantics/openAccess
restricted use
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