| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Needle in a haystack: involvement of the copepod Paracartia grani, in the life-cycle of the oyster pathogen Marteilia refringens
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| Autores: |
Audemard, Corinne
Le Roux, Frédérique
Barnaud, Antoine
Collins, Catherine
Sautour, B
Sauriau, Pierre-guy
De Montaudouin, X
Coustau, C
Combes, C
Berthe, Franck
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| Data: |
2002-09
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| Ano: |
2002
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| Palavras-chave: |
Experimental transmission
In situ hybridization
PCR
Paracartia grani
Ostrea edulis
Marteilia refringens
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| Resumo: |
Marteilia refringens is a major pathogen of the European flat oyster, Ostrea edulis Linnaeus. Since its description, the lifecycle of this protozoan parasite has eluded discovery. Attempts to infect oysters experimentally have been unsuccessful and led to the hypothesis of a complex life-cycle involving several hosts. Knowledge of this life-cycle is of central importance in order to manage oyster disease. However, the exploration of M. refringens life-cycle has been previously limited by the detection tools available and the tremendous number of species to be screened in enzootic areas. In this study, these two restrictions were circumvented by the use of both molecular detection tools and a mesocosm with low biodiversity. Screening of the entire fauna of the pond for AT. refringens DNA was systematically undertaken using PCR. Here, we show that the copepod Paracartia (Acartia) grani is a host of M. refringens. Not only was DNA of M. refringens consistently detectcd in P. grani but also the presence of the parasite in the ovarian tissues was demonstrated using in situ hybridization. Finally, successful experimental transmissions provided evidence that P. grani can be infected from infected flat oysters.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2002/publication-2778.pdf
DOI:10.1017/S0031182001001111
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| Editor: |
Cambridge University Press
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| Relação: |
http://archimer.ifremer.fr/doc/00000/2778/
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| Formato: |
application/pdf
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| Fonte: |
Parasitology (0031-1820) (Cambridge University Press), 2002-09 , Vol. 124 , P. 315-323
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| Direitos: |
Cambridge University Press
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