| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Unveiling protist diversity associated with the Pacific oyster Crassostrea gigas using blocking and excluding primers
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| Autores: |
Clerissi, Camille
Guillou, Laure
Escoubas, Jean Michel
Toulza, Eve
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| Data: |
2020-07
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| Ano: |
2020
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| Palavras-chave: |
Holobiont
Metabarcoding
Microbiome
Ostreoida
Crassostrea
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| Resumo: |
Background Microbiome of macroorganisms might directly or indirectly influence host development and homeostasis. Many studies focused on the diversity and distribution of prokaryotes within these assemblages, but the eukaryotic microbial compartment remains underexplored so far. Results To tackle this issue, we compared blocking and excluding primers to analyze microeukaryotic communities associated with Crassostrea gigas oysters. High-throughput sequencing of 18S rRNA genes variable loops revealed that excluding primers performed better by not amplifying oyster DNA, whereas the blocking primer did not totally prevent host contaminations. However, blocking and excluding primers showed similar pattern of alpha and beta diversities when protist communities were sequenced using metabarcoding. Alveolata, Stramenopiles and Archaeplastida were the main protist phyla associated with oysters. In particular, Codonellopsis, Cyclotella, Gymnodinium, Polarella, Trichodina, and Woloszynskia were the dominant genera. The potential pathogen Alexandrium was also found in high abundances within some samples. Conclusions Our study revealed the main protist taxa within oysters as well as the occurrence of potential oyster pathogens. These new primer sets are promising tools to better understand oyster homeostasis and disease development, such as the Pacific Oyster Mortality Syndrome (POMS) targeting juveniles.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00636/74799/75165.pdf
https://archimer.ifremer.fr/doc/00636/74799/75166.xls
https://archimer.ifremer.fr/doc/00636/74799/75167.tiff
https://archimer.ifremer.fr/doc/00636/74799/75168.xls
https://archimer.ifremer.fr/doc/00636/74799/75169.tiff
https://archimer.ifremer.fr/doc/00636/74799/75170.xls
https://archimer.ifremer.fr/doc/00636/74799/75171.xls
https://archimer.ifremer.fr/doc/00636/74799/75172.tiff
https://archimer.ifremer.fr/doc/00636/74799/75173.xls
DOI:10.1186/s12866-020-01860-1
https://archimer.ifremer.fr/doc/00636/74799/
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| Editor: |
Springer Science and Business Media LLC
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| Relação: |
info:eu-repo/grantAgreement/EC/FP7/678589/EU//VIVALDI
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| Formato: |
application/pdf
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| Fonte: |
Bmc Microbiology (1471-2180) (Springer Science and Business Media LLC), 2020-07 , Vol. 20 , N. 1 , P. 193 (13p.)
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| Direitos: |
info:eu-repo/semantics/openAccess
restricted use
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