| Registro completo |
| Provedor de dados: |
BABT
|
| País: |
Brazil
|
| Título: |
Effects of GDF-9 and FSH on mRNA expression for FSH-R, GDF-9 and BMPs in in vitro cultured goat preantral follicles
|
| Autores: |
Leitão,Cintia Camurça Fernandes
Costa,José Jackson do Nascimento
Brito,Ivina Rocha
Magalhães-Padilha,Déborah de Melo
Almeida,Anderson Pinto
Figueiredo,José Ricardo de
van den Hurk,Robert
Silva,José Roberto Viana
|
| Data: |
2014-04-01
|
| Ano: |
2014
|
| Palavras-chave: |
BMPs
Follicles
GDF-9
In vitro
MRNA
|
| Resumo: |
The aim of the present study was to determine the role of GDF-9 and/or FSH on the growth and mRNA expression for FSH-R, GDF-9, and BMPs in goat secondary follicles after culture in vitro. Goat secondary follicles (~200µm) were isolated and cultured for six days in minimum essential medium (MEM) supplemented with GDF-9 (200 ng/mL), FSH (50 ng/mL) or both. At the beginning and end of culture, the follicular diameter was evaluated and compared. The levels of mRNA for GDF-9, FSH-R and BMPs -2, -4, -6, -7 and -15 in cultured follicles were quantified by real time PCR. The results showed that a significant increase of follicle diameter after six days when compared to day 0, but the presence of GDF-9 and FSH did not influence the follicular growth in comparison with those cultured in MEM. Real time PCR showed that GDF-9 down-regulated the levels of mRNA for BMPs -2 and -15, while FSH either alone or in combination with GDF-9 did not affect the expression of GDF-9, FSH-R and BMPs. In conclusion, GDF-9 reduced the expression of BMP-2 and -15 in caprine preantral follicles after their culture, but FSH either alone or in association with GDF-9 did not control the expression of GDF-9, FSH-R and BMPs.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200007
|
| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
|
| Relação: |
10.1590/S1516-89132014000200007
|
| Formato: |
text/html
|
| Fonte: |
Brazilian Archives of Biology and Technology v.57 n.2 2014
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
|
