| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Improved purification process of β- and α-trypsin isoforms by ion-exchange chromatography
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| Autores: |
Santos,Alexandre Martins Costa
Oliveira,Jamil Silvano de
Bittar,Eustáquio Resende
Silva,Anderson Lourenço da
Guia,Marcos Luiz dos Mares
Bemquerer,Marcelo Porto
Santoro,Marcelo Matos
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| Data: |
2008-08-01
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| Ano: |
2008
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| Palavras-chave: |
Trypsin isoforms
Resolution
Ion-exchange chromatography
Purification
Specific activity
Mass spectrometry
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| Resumo: |
The purpose of this work was to improve the separation and yield of pure β- and α-trypsin isoforms by ion-exchange chromatography and to characterize some physical-chemical properties of these isoforms. Purification of trypsin isoforms was performed by ion-exchange chromatography in 0.1 mol/L tris-HC buffer, pH 7.10 at 4ºC. The sample loading, salt concentration, flow rate and pH of mobile phase were varied to determine their effects on the resolution of the separation. The resolution was optimized mainly between β- and α-trypsin. Pure isoforms were obtained by chromatographying 100 mg of commercial trypsin during seven days, yielding 51 mg of high purity β-trypsin and 13 mg of α-trypsin partially pure, with small amounts of contaminating of ψ-trypsin. Thus, time and resolution of purification were optimized yielding large amounts of pure active enzymes that are useful for several research areas and biotechnology.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400009
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132008000400009
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.51 n.4 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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