| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Biochemical and morphological responses to abiotc elicitor chitin in suspension-cultured sugarcane cells
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| Autores: |
Gallão,Maria Izabel
Cortelazzo,Ângelo Luiz
Fevereiro,Manuel Pedro Salema
Brito,Edy Sousa de
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| Data: |
2010-04-01
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| Ano: |
2010
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| Palavras-chave: |
Chitin
Elicitation
Peroxidase
PAL
Sugarcane
Tissue culture
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| Resumo: |
Cells of Saccharum officinarum submitted to hydrolyzated chitin for 1 to 8h produced phenolic compounds. These alterations were observed through cytochemical methods using Toluidine Blue and Phloroglucinol/HCl. After 4 h, besides cell wall change, there was a change in nuclear pattern of chitin treated cells. There was a 96% increase in nuclear area in 6 h chitin treated material, as observed by Feulgen reaction. The treated cells showed chromatin compacted regions and a degeneration process of nucleoli. In the outer areas of cell wall, there was a polysaccharide desagregation, confirming results obtained for different plants with the use of other elicitors. Peroxidase activity was maximal after 4 h and decreased progressively. PAL activity started to increase at 4 h of incubation. These results showed that chitin hydrolyzate stimulated a defense response in sugarcane cells.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200002
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132010000200002
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.53 n.2 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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