| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Production and characterization of endo-polygalacturonase from Aspergillus niger in solid-state fermentation in double-surface bioreactor
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| Autores: |
Hendges,Diogo Henrique
Montanari,Queli
Malvessi,Eloane
Silveira,Mauricio Moura da
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| Data: |
2011-04-01
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| Ano: |
2011
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| Palavras-chave: |
Aspergillus niger
Double-surface bioreactor
Endo-polygalacturonase
Solid-state fermentation
Enzyme stability
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| Resumo: |
Endo-polygalacturonase (endo-PG) production by Aspergillus niger T0005/007-2 in solid medium with 170 mm of height was evaluated in a cylindrical double surface bioreactor in 96-h experiments. Cell concentration close to 92 mg.g -¹ dm (mg per g of dry medium) in the standard condition (static) was achieved, whereas in tests under forced aeration of 1.4 and 2.8 L.min-1. Kg-1 mm (L of air per minute per Kg of moist medium) and with the central shaft fungal biomass attained approximately 100 mg.g-1 dm. Superior endo-PG activity was obtained with the central-shaft system, 78 U.g-1 dm (units per g of dry medium). Forced aeration and pressure pulse showed no positive effect on the production of endo-PG, 45 U.g-1 dm and 28 U.g-1 dm, respectively. None of the conditions evaluated was efficient for medium temperature control. Endo-PG was stable up to 40ºC. The activity decreased in 50% after 120 minutes at 50ºC, which is a temperature normally found during this process.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200005
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132011000200005
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.54 n.2 2011
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| Direitos: |
info:eu-repo/semantics/openAccess
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