| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Cloning of high molecular weight gluten subunit promoter and study on its function in wheat
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| Autores: |
Weibo,Jin
Jin,Liu
Fangli,Wu
Aiguang,Guo
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| Data: |
2009-04-01
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| Ano: |
2009
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| Palavras-chave: |
Triticum aestivum
Glutenin
Promoter
Endosperm-specific
Transient expression
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| Resumo: |
The aim of this work was to study the cloning and characterization of HMW-GS 1Dx2 promoter from Triticum aestivum. A 1050 bp partial promoter fragment including a putative TATA box and 5' encoding sequence of the gene was cloned by amplifying the upstream sequences using the nest-PCR with appropriate primers. The analysis of the promoter sequence against the PLACE (Plant cis-acting Regulatory DNA Elements) database showed the presence of certain putative endosperm-specific regulatory cis-elements in the sequence along with the TATA and CAAT boxes. The histochemical method detected the transient expressions of GUS in the seeds of wheat. The results showed that HMW-GS 1Dx2 promoter had the endosperm-specific transcription activity in the wheat seeds.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200002
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132009000200002
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.52 n.2 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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