| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Production and characterization of monoclonal antibodies anti fragment Fc of bovine IgG
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| Autores: |
Penha,Tânia Regina
Krüger,Ernesto Renato
Thomaz-Soccol,Vanete
Agottani,Jorge Victor Bacila
Itano,Flávio Hiroshi
Troiano,Ludmilla Della Coletta
Brodzinski,Josiane
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| Data: |
2010-02-01
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| Ano: |
2010
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| Palavras-chave: |
Monoclonal antibodies
Bovine immunoglobulin G
Fragment Fc
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| Resumo: |
The aim of this work was to produce and characterize monoclonal antibodies anti bovine immunoglobulin G (IgG). Out of seven hybridomas, two were chosen based on the ELISA'S absorbance values and were labeled B4F11 and B3H12. These monoclonals were analyzed through Western Blot for IgG fragments obtained by proteolysis with papain, separated by electrophoresis in polyacrylamide gel electrophoresis with β-mercaptoetanol as reducing agent. This revealed that, possibly, the B4F11 was directed to a conformational antigen, and that B3H12 reacted in a specific fashion with Fc (Bovine IgG crystallizable fragment). This antibody could be used in the development of reagents to immunoassays relevant for research and diagnosis.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100014
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132010000100014
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.53 n.1 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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