| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Plant regeneration through somatic embryogenesis of yacón [smallanthus sonchifolius (poepp. and endl.) H. Robinson]
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| Autores: |
Corrêa,Cynthia Manyra
Oliveira,Graciele Nicolodi de
Astarita,Leandro Vieira
Santarém,Eliane Romanato
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| Data: |
2009-06-01
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| Ano: |
2009
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| Palavras-chave: |
Medicinal plant
Tissue culture
Polymnia sonchifolia
Micropropagation
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| Resumo: |
Smallanthus sonchifolius has tuberous roots containing large amounts of fructo-oligosaccharides and its medicinal use has increased due to the hypoglycemic properties reported for this species. An efficient system for propagation via somatic embryogenesis is reported using petiole segments cultivated on MS medium supplemented with combinations of BA, kinetin and 2,4-D, under light and darkness conditions. Embryogenic callus was formed in most of the treatments; however, somatic embryogenesis was promoted by the presence of light. Clusters of somatic embryos appeared on callus surface after 50 days of culture. The highest number of embryos was produced on 0.45 µM BA and 4.5 µM 2,4-D. Embryogenic calli were maintained on MS medium containing 4.5 µM BA and 0.045 µM 2,4-D. Embryos converted on hormone-free half-strength MS medium with 2 g.L-1 activated charcoal and plantlets were transferred to non-sterile conditions for acclimatization, showing 100% of survival.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300005
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132009000300005
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.52 n.3 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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