| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Optimization Medium Composition for Vitamin K2 by Flavobacterium sp. using Response Surface Methodology and Addition of Arachis hypogaea
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| Autores: |
Tan,Mu
Liu,Hui
Li,Zhemin
Sun,Xiaowen
Zheng,Zhiming
Zhao,Genhai
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| Data: |
2016-01-01
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| Ano: |
2016
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| Palavras-chave: |
Arachis hypogaea
D-(+)-catechin
Response surface methodology
Vitamin K2
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| Resumo: |
ABSTRACT The purpose of this research was to enhance the production of vitamin K2 by fermentation optimization and Arachis hypogaea supplementation in Flavobacterium sp. mutant SP-L-01. Optimized culture condition were as follows: 6-days shake-flask culture at 37oC with initial pH value 7.0 ± 0.2, shaking speed in 120 r/min and medium volume of 30 mL with 2% inoculums. After optimization of fermentation medium by response surface methodology (RSM), optimized medium were maltose 23.8 g/l, glucose 9.69 g/l, beef extract 15 g/l, K2HPO4 4.5 g/l，NaCl 3.0 g/l and MgSO4·7H2O 0.3 g/l. Production of vitamin K2 after optimization reached to 10.97 mg/l, which is 79.25% higher than that before optimization (6.12 mg/l). 3 mg/mL of arachis hypogaea was added into the medium at 72 h of shake-flake cultivation, which improved the production of menaquinone-4 (MK4) up to 371% and menaquinone-6 (MK6) up to 149% higher than those of the original medium. D-(+)-catechin, one of the components of arachis hypogaea, was added alone into the medium, which also improved the vitamin K2 synthesis.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100501
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/1678-4324-2016150343
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.59 2016
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| Direitos: |
info:eu-repo/semantics/openAccess
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