| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains
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| Autores: |
Oliveira,Arlem Nascimento de
Oliveira,Luiz Antonio de
Andrade,Jerusa Souza
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| Data: |
2010-02-01
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| Ano: |
2010
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| Palavras-chave: |
Rhizobium
Bradyrhizobium
Amylase production
Enzymatic properties
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| Resumo: |
Amylase production and partial characterization of crude enzyme preparations from two rhizobia strains (R-926 and R-991) were evaluated. For both the strains, maximal amylase activities were achieved during the early-to-mid- exponential growth phase; both were active over a pH range from 4.5 to 8.5 and temperature from 30 to 50 ºC. None of the ions studied (K+, Na+, Ca2+, Hg2+, Mg2+, Mn2+, Cu2+ and Zn2+) was required for the catalytic activity of strain R-926; amylase activity of strain R-991 was stimulated in the presence of K+, Hg2+ and Zn2+. The surfactants SDS, Triton X-100 and Tween-80 did not have a pronounced inhibitory effect on enzyme activities; SDS and Tween-80 caused the highest stimulatory effects. Amylase activities from the rhizobia strains were reduced by up to 30% in the presence of EDTA; amylase activity of R-926 was also inhibited by HgCl2, suggesting that Ca2+and cysteine residues could be important for activity of this strain.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100005
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132010000100005
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.53 n.1 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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