| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Purification and characterization of an alpha-galactosidase from Aspergillus fumigatus
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| Autores: |
Rezende,Sebastião Tavares de
Guimarães,Valéria Monteze
Rodrigues,Marília de Castro
Felix,Carlos Roberto
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| Data: |
2005-03-01
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| Ano: |
2005
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| Palavras-chave: |
Aspergillus fumigatus
Alpha-galactosidase
Raffinose oligosaccharides
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| Resumo: |
Aspergillus fumigatus secreted invertase (beta-fructofuranosidase) and alpha-galactosidase enzymatic activities able to hydrolyzing raffinose oligosaccharides (RO). alpha-Galactosidase was induced by galactose, melibiose and raffinose, but galactose was the most efficient inducer. It was purified by gel filtration and two ion exchange chromatographies and showed Mw of 54.7 kDa. The purified enzyme showed maximal activity against p-nitrophenyl-alpha-D-galactopyranoside (pNPGal) at pH 4.5-5.5 and 55 °C, and retained about 80% of the original activity after incubation for 90 minutes at 50ºC. The KM for pNPGal was 0.3 mM. Melibiose was hydrolyzed by the enzyme but raffinose was very poor substrate.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200005
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132005000200005
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.48 n.2 2005
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| Direitos: |
info:eu-repo/semantics/openAccess
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