| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4
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| Autores: |
Acevedo,Francisca
Pizzul,Leticia
Castillo,María del Pilar
Rubilar,Olga
Lienqueo,María Elena
Tortella,Gonzalo
Diez,María Cristina
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| Data: |
2011-12-01
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| Ano: |
2011
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| Palavras-chave: |
White-rot fungi
Ligninolytic enzymes
Purification
Lignocellulosic material
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| Resumo: |
In this study, different growth conditions of Anthracophyllum discolor Sp4 including the effect of agitation, additions of lignocellulosic support, inducer and surfactant were evaluated on the MnP production in Kirk medium using a culture system made up of the tubes containing the glass bead . The highest MnP production (1,354 U/L on day 13) was obtained when the medium was supplemented with wheat grain and 0.25 mM MnSO4 as inducer, under static conditions at 30°C. Two isoenzymes were purified (35 and 38 kDa respectively). MnP presented a maximal activity in the pH range between 4.5 and 5.5, a relatively high temperature tolerance (50ºC) and a high catalytic activity for 2,6-dimethoxyphenol and hydrogen peroxide.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600013
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132011000600013
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.54 n.6 2011
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| Direitos: |
info:eu-repo/semantics/openAccess
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