| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Validation of an ovarian biopsy method for monitoring oocyte development in the fat snook, Centropomus parallelus Poey, 1860 in captivity
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| Autores: |
Ferraz,Eduardo Medeiros
Alvarez-Lajonchère,Luis
Cerqueira,Vinicius Ronzani
Candido,Sidinei
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| Data: |
2004-08-01
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| Ano: |
2004
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| Palavras-chave: |
Ovarian Biopsy
Fat Snook
Centropomus parallelus
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| Resumo: |
The validation of an ovarian biopsy method for in vivo assessment of oocyte maturation in Centropomus parallelus was studied. Diameters of intra-ovarian oocytes siphoned with cannula were analyzed fresh and preserved with 1% formalin in 0.7% NaCl solution. Oocytes in different stages were present along the ovaries, up to the tertiary yolk globule stage, which had a unimodal diameter frequency distribution. The oocyte diameter means were not significantly different at four sites along the ovaries (P > 0.05). Samples obtained with cannula were representative of the ovary central portion, in vivo and in vitro samples of the seven females examined were not significantly different (P > 0.05). An estimate of the coefficient of variation corrected for bias (P < 0.05) for 8 repeated in vivo samples was 1.9 ± 0.6. The results demonstrated that for the species, the biopsy method was satisfactory, providing representative samples of the ovaries.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400018
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132004000400018
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.47 n.4 2004
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| Direitos: |
info:eu-repo/semantics/openAccess
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