| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Cell density and solvent are critical parameters affecting formazan evaluation in MTT assay
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| Autores: |
Gasque,Kellen Cristina da Silva
Al-Ahj,Luana Polioni
Oliveira,Rodrigo Cardoso
Magalhães,Ana Carolina
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| Data: |
2014-06-01
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| Ano: |
2014
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| Palavras-chave: |
Cell viability
MTT assay
Fibroblast NIH/3T3
Solvents
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| Resumo: |
The aim of this study was to establish the more accurate protocol for fibroblast cell viability using MTT assay. NIH/3T3 fibroblasts were seeded at the following cell densities: 3.125x10³; 1.156x10(4); 3.125x10(4); 1.156x10(5) and 3.125x10(5) cells/cm². Following 24h of seeding, MTT was added to the wells. After 4h of the MTT addition, different solvents were added to solubilize the formazan crystals: 1) HCl/SDS group- 20% SDS and 0.01 M HCl; 2) EtOH/ HAc group-50% ethanol and 1% acetic acid; 3) DMSO group- 99.5% dimethyl sulfoxide; and 4) PropOH group- 99.5% isopropanol. The absorbance values were measured using a spectrophotometer at 570 nm. The data were analyzed by 2-way ANOVA (p<0.05) and showed that the absorbance average varied according to the number of cells and solvents: HCl/SDS (0 to 0.13), EtOH/HAc (0 to 0.22), DMSO (0.76 to 1.31) and PropOH (0.66 to 1.04). The DMSO and PropOH groups presented the most appropriate protocols for NIH/3T3 fibroblasts cell viability, especially at the density of 1.156x10(4) cells/cm².
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300011
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132014005000007
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.57 n.3 2014
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| Direitos: |
info:eu-repo/semantics/openAccess
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