| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Evaluation of Antimicrobial Activity of Glucose Oxidase from Aspergillus niger EBL-A and Penicillium notatum
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| Autores: |
Zia,Muhammad Anjum
Riaz,Ayesha
Rasul,Samreen
Abbas,Rao Zahid
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| Data: |
2013-12-01
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| Ano: |
2013
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| Palavras-chave: |
Glucose oxidase
Antimicrobial
Aspergillus niger
Penicillium notatum
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| Resumo: |
This work aimed to study the production and purification of glucose oxidase by Aspergillus niger and Penicillium notatum using corn steep liquor as the substrate and evaluate its antimicrobial activity for use in pharmaceutical and food industries. The enzyme was purified by ammonium sulfate precipitation (60-85%), DEAE-cellulose ion exchange and Sephadex G-200 size exclusion chromatography. The crude enzyme extracts of A. niger and P. notatum showed 2.32 and 5.53 U mg-1 specific activities, respectively, which after desalting was 15.52 and 12.05 U mg-1, and after ion exchange and gel filtration chromatography was 29.09 - 62 and 25.72 - 59.37 U mg-1 for A. niger and P. notatum, respectively. The antimicrobial activity was determined by disc diffusion method against selected microbial strains where glucose oxidase from A. niger showed anti-bacterial activity, while no fungicidal effects were shown by both A. niger and P. notatum glucose oxidases.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600010
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132013005000010
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.56 n.6 2013
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| Direitos: |
info:eu-repo/semantics/openAccess
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