| Registro completo |
| Provedor de dados: |
BABT
|
| País: |
Brazil
|
| Título: |
Extra-cellular isoamylase production by Rhizopus oryzae in solid-state fermentation of agro wastes
|
| Autores: |
Ghosh,Barnita
Ray,Rina Rani
|
| Data: |
2011-10-01
|
| Ano: |
2011
|
| Palavras-chave: |
Isoamylase
Rhizopus oryzae
SSF
Waste utilization
|
| Resumo: |
Extra-cellular isoamylase was produced by Rhizopus oryzae PR7 in solid-state fermentations of various agro wastes, among which millet, oat, tapioca, and arum (Colocasia esculenta) showed promising results. The highest amount of enzyme production was obtained after 72 h of growth at 28°C. The optimum pH for enzyme production was - 8.0. Among the various additives tested, enzyme production increased with ions such as Ca2+, Mg2+ and also with cysteine, GSH, and DTT. The enzyme synthesis was reduced in the presence of thiol inhibitors like Cu2+ and pCMB. The surfactants like Tween-40, Tween-80 and Triton X-100 helped in enhancing the enzyme activity. The production could be further increased by using the combinations of substrates. The ability to produce high amount of isoamylase within a relatively very short period and the capability of degrading wastes could make the strain suitable for commercial production of the enzyme.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500003
|
| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
|
| Relação: |
10.1590/S1516-89132011000500003
|
| Formato: |
text/html
|
| Fonte: |
Brazilian Archives of Biology and Technology v.54 n.5 2011
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
