| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol
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| Autores: |
Singh,Jagdish
Verma,Neelam
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| Data: |
2010-10-01
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| Ano: |
2010
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| Palavras-chave: |
Aspergillus niger
Glucose oxidase
Aqueous two phase
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| Resumo: |
The aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l-1), potassium phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2%) was recovered in polymer phase by 11.8 fold purification, giving a yield of 129U mg protein-¹.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500007
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132010000500007
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.53 n.5 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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