| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Production of pectinases by A. niger: influence of fermentation conditions
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| Autores: |
Martos,María A.
Martinez Vazquez,Francisco
Benassi,Fernando O.
Hours,Roque A.
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| Data: |
2009-06-01
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| Ano: |
2009
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| Palavras-chave: |
Pectinases
Polygalacturonase
Pectinesterase
Aspergillus niger
Response surface methodology
Citrus pectin
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| Resumo: |
Response surface methodology was used for optimization of polygalacturonase (PG) and pectinesterase (PE) production in submerged fermentation by A.niger. A Central Composite Experimental Design was applied, consisting of 22 experiments, including eight central points. Variables studied were: fermentation time (24 to 120 h), pH (3.5 to 6.5) and initial concentration of pectin (5 to 20 g/l). Maximum PE production was 220 U/l, after 74 h of culture, in a medium containing 20 g/l of pectin (pH 6.5). The optimal conditions for PG production were pH: 4.1, 20 g/l of pectin and 94 h of fermentation with a maximum value of 1032 U/l. Under these conditions, the PE production was low (15 U/l). A liquid extract with high PG activity and low PE activity could be suitable to be used in food processing in order to reduce the production of methanol.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300007
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132009000300007
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.52 n.3 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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