| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Oxidative Stress, Chromium-Resistance and Uptake by Fungi: Isolated from Industrial Wastewater
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| Autores: |
Elahi,Amina
Rehman,Abdul
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| Data: |
2017-01-01
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| Ano: |
2017
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| Palavras-chave: |
Trichosporon asahii
Rhodotorula mucilaginosa
Wastewater
Resistance
Bioaccumulation
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| Resumo: |
ABSTRACT Trichosporon asahii and Rhodotorula mucilaginosa isolated from wastewater effluents were identified as chromium-resistant yeasts. Cr(VI) concentrations at 8 mM and 6 mM were inhibitory for R. mucilaginosa and T. asahii. Remarkably elevated GSH (69.88 ± 10.01) and GSSG (11.24 ± 0.96) was observed under metal stress in T. asahii as compared to R. mucilaginosa GSH (18.95 ± 3.19) and GSSG (3.7 ± 2.74) mM g-1 8 level. Statistical analysis revealed significantly higher GSH/GSSG ratio in both strains. NPSH (29.84 ± 0.54) level in T. asahii was much higher than in R. mucilaginosa (6.05 ± 0.24). Chromate reductase (ChR) was assayed and its activity was optimum at 50°C (pH 6) in T. asahii while R. mucilaginosa showed higher activity at 30°C (pH 7). Activity of both ChRs was enhanced in the presence of Mg, Na, Co and Ca but strongly inhibited by Hg cations. Cr(VI) uptake capabilities were ranged between 43-97% in R. mucilaginosa and 35-88% in T. asahii. One dimensional electrophoresis revealed enriched bands of cysteine rich metallothioneins suggesting some differential proteins could be overexpressed under Cr(VI) stress.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100452
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/1678-4324-2017160394
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.60 2017
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| Direitos: |
info:eu-repo/semantics/openAccess
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