| Registro completo |
| Provedor de dados: |
BABT
|
| País: |
Brazil
|
| Título: |
Semliki forest virus as a vector: pros and cons for its use in biopharmaceuticals production
|
| Autores: |
Núñez,Eutimio Gustavo Fernández
Jorge,Soraia Attie Calil
Astray,Renato Mancini
Rezende,Alexandre Gonçalves de
Costa,Bruno Labate Vale da
Monteiro,Daniella Cristina Ventini
Pereira,Carlos Augusto
Tonso,Aldo
|
| Data: |
2013-10-01
|
| Ano: |
2013
|
| Palavras-chave: |
BHK-21
Large-scale bioprocesses
Mammalian cells
Recombinant proteins
SFV
Transient expression
|
| Resumo: |
The number of biopharmaceuticals for medical and veterinarian use produced in mammalian cells is increasing year after year. All of them are obtained by stable recombinant cell lines. However, it is recognized that transient gene expression produces high level expression in a short time. In that sense, viral vectors have been extensively used for producing recombinant proteins on lab-scale. Among them, Semliki Forest virus is commonly employed for this purpose. This review discusses the main aspects related to the use of Semliki Forest virus technology as well as its advantages and drawbacks which limit currently its utilization in biopharmaceutical industry on large-scale.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500018
|
| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
|
| Relação: |
10.1590/S1516-89132013000500018
|
| Formato: |
text/html
|
| Fonte: |
Brazilian Archives of Biology and Technology v.56 n.5 2013
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
