| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Production and regeneration of protoplasts from orchid Mycorrhizal Fungi Epulorhiza repens and Ceratorhiza sp.
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| Autores: |
Coelho,Irene da Silva
Queiroz,Marisa Vieira de
Costa,Maurício Dutra
Kasuya,Maria Catarina Megumi
Araújo,Elza Fernandes de
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| Data: |
2010-02-01
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| Ano: |
2010
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| Palavras-chave: |
Ceratorhiza
Epulorhiza
Molecular biology
Protoplast
Regeneration
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| Resumo: |
The aim of this work was to study the standardization of conditions to obtain and regenerate Epulorhiza repens and Ceratorhiza sp. protoplasts. For E. repens, the largest number of protoplasts (8.0 × 10(6) protoplasts/mL) was obtained in 0.6 M KCl, using 15 mg/mL of Lysing Enzymes, and 2-day-old fungal mycelium. When 0.5 M sucrose was used as osmotic stabilizer, the highest frequency of regeneration was achieved (8.5 %); 80.0 % of protoplasts were nucleated, and 20.0 % anucleated. For Ceratorhiza sp., the largest number of protoplasts (4.0 × 10(7) protoplasts/mL) was achieved in 0.6 M NaCl, when 15 mg/mL of Lysing Enzymes and 15mg/mL of Glucanex, with 2-day-old fungal mycelium were used. The highest frequency of regeneration was 6.7 % using 0.5 M sucrose as osmotic stabilizer; 88.8 % of protoplasts were nucleated, and 11.2 % anucleated.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100019
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132010000100019
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.53 n.1 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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