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Provedor de dados: |
BABT
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País: |
Brazil
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Título: |
Effect of the medium composition on formation of amylase by Bacillus sp
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Autores: |
Santos,Eliana de Oliveira.
Martins,Meire Lelis Leal
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Data: |
2003-01-01
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Ano: |
2003
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Palavras-chave: |
Alpha-amylase
Thermophilic bacterium
Bacillus sp
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Resumo: |
Studies on the alpha -amylase synthesis was carried out with a moderately thermophilic, facultatively anaerobic Bacillus sp, isolated from soil samples. The cells were cultivated in a complex medium containing soluble starch or maltose as carbon source. The levels of the alpha -amylaseactivity detected in culture supernatants varied greatly with the type of carbon source used. Maltose, soluble starch and citrate stimulated alpha -amylaseformation. Addition of exogenous glucose repressed formation of alpha -amylase, demonstrating that a classical glucose effect was operative in this organism. The concentration of yeast extract was found to be important factor in the alpha -amylase synthesis bythe isolate.The activity of the enzyme increased between 2 and 5 g/L yeast extract concentration and then fell very rapidly beyond this point. The best concentration of peptone to alpha-amylase formation was found to be around 10g/L.
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Tipo: |
Info:eu-repo/semantics/article
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Idioma: |
Inglês
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Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100018
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Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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Relação: |
10.1590/S1516-89132003000100018
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Formato: |
text/html
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Fonte: |
Brazilian Archives of Biology and Technology v.46 n.1 2003
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Direitos: |
info:eu-repo/semantics/openAccess
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