| Registro completo |
| Provedor de dados: |
BJID
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| País: |
Brazil
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| Título: |
Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients
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| Autores: |
Carraro,Emerson
Pasternak,Jacyr
Perosa,Ana
Siqueira,Itacy
Martino,Marines Dalla Vale
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| Data: |
2010-06-01
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| Ano: |
2010
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| Palavras-chave: |
Cytomegalovirus
Antigenemia assay
Immmunoperoxidase
Immunofluorescence
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| Resumo: |
Cytomegalovirus (CMV) antigenemia is still one of the two major assays available for diagnosis and monitoring of CMV infections. A commercial rapid test recently available in Brazil for quantification of human cytomegalovirus pp65 antigenemia revealed by immunofluorescence technique was compared with the original in-house method revealed by immunoperoxidase in patients receiving solid organ transplants. Of 80 blood samples tested for CMV antigenemia, 34 (42.5%) were positive: commercial assay detected 33 (97%) and in-house assay detected 20 (58.8%) samples. The numbers of positive cells in the two assays were different, with a median of 4.5 and 12 positive cells obtained by in-house and commercial kit, respectively. Discrepancies between assays occurred in 15 specimens from patients with low-grade antigenemia (median 6 positive cells). The assay-time was reduced in approximately 50% compared to in-house methodology. In conclusion, besides comparable results obtained for both assays, the commercial antigenemia assay provides more rapid and sensitive results.
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| Tipo: |
Info:eu-repo/semantics/other
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000300023
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| Editor: |
Brazilian Society of Infectious Diseases
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| Relação: |
10.1590/S1413-86702010000300023
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Infectious Diseases v.14 n.3 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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