| Registro completo |
| Provedor de dados: |
BJID
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| País: |
Brazil
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| Título: |
Human papillomavirus detection and typing using a nested-PCR-RFLP assay
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| Autores: |
Coser,Janaina
Boeira,Thaís da Rocha
Fonseca,André Salvador Kazantzi
Ikuta,Nilo
Lunge,Vagner Ricardo
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| Data: |
2011-10-01
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| Ano: |
2011
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| Palavras-chave: |
DNA probes
HPV polymerase chain reaction polymorphism
Restriction fragment length
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| Resumo: |
BACKGROUND: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to detect and type HPV based on the analysis of L1 gene. METHODS: Analysis of published DNA sequence of mucosal HPV types to select sequences of new primers. Design of an original nested-PCR assay using the new primers pair selected and classical MY09/11 primers. HPV detection and typing in cervical samples using the nested-PCR-RFLP assay. RESULTS: The nested-PCR-RFLP assay detected and typed HPV in cervical samples. Of the total of 128 clinical samples submitted to simple PCR and nested-PCR for detection of HPV, 37 (28.9%) were positive for the virus by both methods and 25 samples were positive only by nested-PCR (67.5% increase in detection rate compared with single PCR). All HPV positive samples were effectively typed by RFLP assay. CONCLUSION: The method of nested-PCR proved to be an effective diagnostic tool for HPV detection and typing.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500009
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| Editor: |
Brazilian Society of Infectious Diseases
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| Relação: |
10.1590/S1413-86702011000500009
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Infectious Diseases v.15 n.5 2011
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| Direitos: |
info:eu-repo/semantics/openAccess
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