| Registro completo |
| Provedor de dados: |
BJID
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| País: |
Brazil
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| Título: |
Development and validation of a simple and rapid way to generate low volume of plasma to be used in point-of-care HIV virus load technologies
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| Autores: |
Vasconcellos,Isabelle
Mariani,Diana
Azevedo,Marcelo C.V.M. de
Ferreira Jr.,Orlando C.
Tanuri,Amilcar
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| Data: |
2020-02-01
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| Ano: |
2020
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| Palavras-chave: |
POC VL
HIV viral load
HIV
Point-of-care
Fingerstick-plasma
MPIMA
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| Resumo: |
ABSTRACT A new point-of-care HIV viral load, mPIMA HIV-1/2 VL, Abbott, USA, has been recently developed. This point-of-care viral load requires no skilled person to run and uses a small plasma volume (50 µL). However, obtaining 50 µL of plasma can be a challenge in limited resource settings. We validated a simple and easy method to obtain enough amount of plasma to run a point-of-care viral load. The study utilized 149 specimens from patients failing antiretroviral therapy. At least 250 µL of whole blood was collected in a microtube/EDTA from fingerstick (fs-plasma) and immediately centrifuged. Parallel collection of venous blood to obtain plasma (vp-plasma) was used to compare performance in a point-of-care viral load assay and in methodology used in centralized laboratories Abbott M2000, Abbott, USA. The procedure for plasma collection takes less than 10 min and in 94% of the cases only one fingerstick was sufficient to collect at least 250 µL of blood. The Pearson correlation coefficient value for vp-plasma versus fs-plasma ran on mPIMA was 0.990. The Bland-Altman mean difference (md) for this comparison were virtually zero (md = −0.001) with limits of agreement between −0.225 and 0.223. In addition, the Pearson correlation coefficient value for fs-plasma in mPIMA versus vp-plasma in Abbott M2000 was 0.948 for values above the mPIMA limit of quantification (LoQ; from 800 to 1,000,000 copies/mL). These results validate this simple plasma isolation method capable to be implemented in low resource countries where point-of-care decentralization is deeply needed.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100030
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| Editor: |
Brazilian Society of Infectious Diseases
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| Relação: |
10.1016/j.bjid.2019.10.010
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Infectious Diseases v.24 n.1 2020
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| Direitos: |
info:eu-repo/semantics/openAccess
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